In vitro Shoot Multiplication of Vernonia cinerea ( L . ) Less . – an important medicinal

A rapid and efficient protocol was developed for inducing direct organogenesis using shoot tip explants of Vernonia cinerea L. Shoot tip explants were cultured on MS medium supplemented with different concentrations of BAP (2.22 to 22.20 μM/l) and KN (2.42 to 23.20 μM/l) for direct shoot induction. The frequency of multiple shoot induction and proliferation increased with increasing concentration of BAP (13.32 μM/l) and KN (13.92 μM/l) at optimal level. The high frequency of multiple shoot proliferation was observed on MS medium containing 13.32 μM/l BAP and 13.92 μM/l KN. The regenerated shoots were successfully rooted on MS medium supplemented with IBA 7.38 μM/l, after sequential hardening, survival rate was 90 %. Key works: Vernonia cinerea, Shoot tip, Direct organogenesis *Corresponding author, E-Mail: [email protected]